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dc.contributor.author Wallinger, Corinna
dc.contributor.author Staudacher, Karin
dc.contributor.author Schallhart, Nikolaus
dc.contributor.author Peter, Eva
dc.contributor.author Dresch, Phillipp
dc.contributor.author Juen, A
dc.contributor.author Traugott, Michael
dc.coverage.spatial Tyrol
dc.date.accessioned 2012-10-12T20:06:37Z
dc.date.available 2012-10-12T20:06:37Z
dc.date.issued 2012-11-20
dc.identifier doi:10.5061/dryad.p7c8s
dc.identifier.citation Wallinger C, Staudacher K, Schallhart N, Peter E, Dresch P, Juen A, Traugott M (2012) The effect of plant identity and the level of plant decay on molecular gut content analysis in a herbivorous soil insect. Molecular Ecology Resources 13(1): 75–83.
dc.identifier.uri http://hdl.handle.net/10255/dryad.42722
dc.description Plant roots represent an important food source for soil-dwelling animals, but tracking herbivore food choices below-ground is difficult. Here, we present an optimized PCR assay for the detection of plant DNA in the guts of invertebrates, using general plant primers targeting the trnT-F chloroplast DNA region. Based on this assay, we assessed the influence of plant identity on the detectability of ingested plant DNA in Agriotes click beetle larvae. Six different plant species were fed to the insects, comprising a grass, a legume, and four non-legume forbs. Moreover, we examined whether it is possible to amplify DNA of decaying plants and if DNA of decayed plant food is detectable in the guts of the larvae. DNA of the ingested roots could be detected in the guts of the larvae for up to 72 h post-feeding, the maximum digestion time tested. When fed with living plants, DNA detection rates differed significantly between the plant species. This may be ascribed to differences in the amount of plant tissue consumed, root palatability, root morphology and/or secondary plant components. These findings indicate that plant identity can affect post-feeding DNA detection success, which needs to be considered for the interpretation of molecularly derived feeding rates on plants. Amplification of plant DNA from decaying plants was possible as long as any tissue could be retrieved from the soil. The consumption of decaying plant tissue could also be verified by our assay, but the insects seemed to prefer fresh roots over decaying plant material.
dc.relation.haspart doi:10.5061/dryad.p7c8s/1
dc.relation.isreferencedby doi:10.1111/1755-0998.12032
dc.relation.isreferencedby PMID:23167731
dc.subject Agriculture
dc.subject Diet Analysis
dc.subject Environmental DNA
dc.subject Foodwebs
dc.subject Insects
dc.subject Invertebrates
dc.subject Herbivory
dc.title Data from: The effect of plant identity and the level of plant decay on molecular gut content analysis in a herbivorous soil insect
dc.type Article *
dwc.ScientificName Triticum aestivum
dwc.ScientificName Zea mays
dwc.ScientificName Pimpinella major
dwc.ScientificName Lolium perenne
dwc.ScientificName Trifolium pratense
dwc.ScientificName Achillea millefolium
dwc.ScientificName Taraxacum officinale
dwc.ScientificName Plantago lanceolata
dwc.ScientificName Agriotes ustulatus
dwc.ScientificName Agriotes obscurus
dwc.ScientificName Agriotes sputator
dwc.ScientificName Agriotes sordidus
dwc.ScientificName Agriotes lineatus
dwc.ScientificName Agriotes proximus
dc.contributor.correspondingAuthor Wallinger, Corinna
prism.publicationName Molecular Ecology Resources

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