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dc.contributor.author Fujino, Naoya
dc.contributor.author Ota, Chiharu
dc.contributor.author Takahashi, Toru
dc.contributor.author Suzuki, Takaya
dc.contributor.author Yamada, Mitsuhiro
dc.contributor.author Nagatomi, Ryouichi
dc.contributor.author Kondo, Takashi
dc.contributor.author Yamaya, Mutsuo
dc.contributor.author Kubo, Hiroshi
dc.date.accessioned 2012-11-12T19:18:28Z
dc.date.available 2012-11-12T19:18:28Z
dc.date.issued 2012-10-31
dc.identifier doi:10.5061/dryad.mc4s1
dc.identifier.citation Fujino N, Ota C, Takahashi T, Suzuki T, Yamada M, Nagatomi R, Kondo T, Yamaya M, Kubo H (2012) Gene expression profiles of alveolar type II cells of chronic obstructive pulmonary disease: a case-control study. BMJ Open 2: e001553.
dc.identifier.uri http://hdl.handle.net/10255/dryad.42958
dc.description Objectives: The aim of this study was to identify the gene expression pattern specific in alveolar epithelial type II cells (ATII cells) isolated from patients with chronic obstructive pulmonary disease (COPD). Design: Case control Setting: Two hospitals in Japan Participants: Three patients without COPD and three patients with COPD in microarray analyses. Five smokers without COPD and nine smokers with COPD in the following analyses. Primary and secondary outcome measured: Primary outcome included identification of differentially expressed genes and activated or inhibited pathways in ATII cells of the patients with COPD, compared to those of the patients without COPD, using Affymetrix gene expression arrays. Secondary outcome included validation of the results of microarray analyses by quantitative reverse transcription-polymerase chain reaction. Results: We isolated ATII cells from COPD and non-COPD lungs using fluorescence-activated cell sorting. We performed Affymetrix gene expression arrays on both types of ATII cells. Gene set enrichment analyses revealed that two major gene sets were enriched in ATII cells from COPD lungs: interferon-responsive gene sets and gene sets associated with cell cycle progression. Gene ontology term enrichment analyses indicated that among the interferon-stimulated genes, ATII cells in COPD expressed genes such as PSMB8, PSMB9, TAP1 and TAP2 associated with the antigen processing and presentation pathway. We validated the results of the microarray analyses using quantitative reverse transcriptase-polymerase chain reaction. In addition, FACS analysis indicated that the percentage of ATII cells to CD45-negative lung cells isolated from COPD lungs were significantly increased more than that from non-COPD lungs. Conclusions: Our study demonstrated that interferon-stimulated genes involved in the antigen processing and presentation pathway and genes involved in cell cycle progression were enriched in ATII cells of the patients with COPD. These pathways might alter phenotypes of ATII cells in COPD lungs.
dc.relation.haspart doi:10.5061/dryad.mc4s1/1
dc.relation.isreferencedby doi:10.1136/bmjopen-2012-001553
dc.relation.isreferencedby PMID:23117565
dc.subject Chronic airways disease
dc.subject Cell biology
dc.subject MOLECULAR BIOLOGY
dc.subject RESPIRATORY MEDICINE (see Thoracic Medicine)
dc.subject COPD
dc.subject alveolar type II epithelial cell
dc.subject gene expression
dc.subject human
dc.title Data from: Gene expression profiles of alveolar type II cells of chronic obstructive pulmonary disease: a case-control study
dc.type Article *
dc.contributor.correspondingAuthor Kubo, Hiroshi
prism.publicationName BMJ Open

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Title GEO GSE29133
Description Link to GEO. Primary ATII cells were isolated from lung tissues obtained from thoracic surgery using FACS. We profiled the gene expression of ATII cells from three non-COPD and three COPD patients. This study was approved by the Ethics Committee at Tohoku University School of Medicine and Ishinomaki Red Cross Hospital. All subjects gave informed consent.
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