Show simple item record García-Verdugo, Carlos Calleja, Juan Antonio Vargas, Pablo Silva, Luis Moreira, Orlanda Pulido, Fernando 2012-12-06T20:19:07Z 2012-12-06T20:19:07Z 2013-02-04
dc.identifier doi:10.5061/dryad.g9s2k
dc.identifier.citation García-Verdugo C, Calleja JA, Vargas P, Silva L, Moreira O, Pulido F (2013) Polyploidy and microsatellite variation in the relict tree Prunus lusitanica L.: how effective are refugia in preserving genotypic diversity of clonal taxa? Molecular Ecology 22(6): 1546–1557.
dc.description Refugia are expected to preserve genetic variation of relict taxa, especially in polyploids, because high gene dosages could prevent genetic erosion in small isolated populations. However, other attributes linked to polyploidy, such as asexual reproduction, may strongly limit the levels of genetic variability in relict populations. Here, ploidy levels and patterns of genetic variation at nuclear microsatellite loci were analysed in Prunus lusitanica, a polyploid species with clonal reproduction that is considered a paradigmatic example of a Tertiary relict. Sampling in this study considered a total of 20 populations of three subspecies: mainland lusitanica (Iberian Peninsula and Morocco), and island azorica (Azores) and hixa (Canary Islands and Madeira). Flow cytometry results supported an octoploid genome for lusitanica and hixa, whereas a 16-ploid level was inferred for azorica. Fixed heterozygosity of a few allele variants at most microsatellite loci resulted in levels of allelic diversity much lower than those expected for a high-order polyploid. Islands as a whole did not contain higher levels of genetic variation (allelic or genotypic) than mainland refuges, but island populations displayed more private alleles and higher genotypic diversity in old volcanic areas. Patterns of microsatellite variation were compatible with the occurrence of clonal individuals in all but two island populations, and the incidence of clonality within populations negatively correlated with the estimated timing of colonization. Our results also suggest that gene flow has been very rare among populations, and thus population growth following founder events was apparently mediated by clonality rather than seed recruitment, especially in mainland areas. This study extends to clonal taxa the idea of oceanic islands as important refugia for biodiversity, since the conditions for generation and maintenance of clonal diversity (i.e. occasional events of sexual reproduction, mutation and/or seed immigration) appear to have been more frequent in these enclaves than in mainland areas.
dc.relation.haspart doi:10.5061/dryad.g9s2k/1
dc.relation.haspart doi:10.5061/dryad.g9s2k/2
dc.relation.haspart doi:10.5061/dryad.g9s2k/3
dc.relation.isreferencedby doi:10.1111/mec.12194
dc.relation.isreferencedby PMID:23379976
dc.subject Angiosperms
dc.subject Population Dynamics
dc.subject Population Genetics - Empirical
dc.subject Phylogeography
dc.title Data from: Polyploidy and microsatellite variation in the relict tree Prunus lusitanica L.: how effective are refugia in preserving genotypic diversity of clonal taxa?
dc.type Article
dwc.ScientificName Prunus lusitanica
dc.contributor.correspondingAuthor García-Verdugo, Carlos
prism.publicationName Molecular Ecology

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Title Prunus_lusitanica_Table S4
Downloaded 56 times
Description Sampled individuals, populations and vouchers
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Title Prunus_lusitanica_nSSR
Downloaded 37 times
Description Microsatellite data for P. lusitanica individuals. Data follows FDASH software format. Columns are separated by TABs. Alleles are separated by "," whereas "?" indicates missing data. Due to the polyploid constitution of these taxa, multiple alleles per individual and locus were scored, but fixed heterozygosity of a few allele variants can be observed at several loci.
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Title Prunus_lusitanica-flow cytometry
Downloaded 19 times
Description 2C values obtained through FCM for a subset of individuals representing P. lusitanica subspecies
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