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dc.contributor.author Alberdi, Antton
dc.contributor.author Garin, Inazio
dc.contributor.author Aizpurua, Ostaizka
dc.contributor.author Aihartza, Joxerra
dc.coverage.spatial Pyrenees
dc.date.accessioned 2012-12-05T18:28:30Z
dc.date.available 2012-12-05T18:28:30Z
dc.date.issued 2012-04-24
dc.identifier doi:10.5061/dryad.611310kt
dc.identifier.citation Alberdi A, Garin I, Aizpurua O, Aihartza J (2012) The foraging ecology of the Mountain long-eared bat Plecotus macrobullaris revealed with DNA mini-barcodes. PLoS ONE 7(4): e35692.
dc.identifier.uri http://hdl.handle.net/10255/dryad.38781
dc.description Molecular analysis of diet overcomes the considerable limitations of traditional techniques for identifying prey remains in bat faeces. We collected faeces from individual Mountain long-eared bats Plecotus macrobullaris trapped using mist nets during the summers of 2009 and 2010 in the Pyrenees. We analysed their diet using DNA mini-barcodes to identify prey species. In addition, we inferred some basic features of the bat’s foraging ecology that had not yet been addressed. P. macrobullaris fed almost exclusively on moths (97.8%). As prey we detected one dipteran genus (Tipulidae) and 29 moth taxa: 28 were identified at species level (23 Noctuidae, 1 Crambidae, 1 Geometridae, 1 Pyralidae, 1 Sphingidae, 1 Tortricidae), and one at genus level (Rhyacia sp., Noctuidae). Known ecological information about the prey species allowed us to determine that bats had foraged at elevations between 1,500 and 2,500 m amsl (above mean sea level), mostly in subalpine meadows, followed by other open habitats such as orophilous grasslands and alpine meadows. No forest prey species were identified in the diet. As 96.4% of identified prey species were tympanate moths and no evidence of gleaning behaviour was revealed, we suggest P. macrobullaris probably forages by aerial hawking using faint echolocation pulses to avoid detection by hearing moths. As we could identify 87.8% of the analysed sequences (64.1% of the MOTUs, Molecular Operational Taxonomic Units) at species level, we conclude that DNA mini-barcodes are a very useful tool to analyse the diet of moth-specialist bats.
dc.relation.haspart doi:10.5061/dryad.611310kt/1
dc.relation.isreferencedby doi:10.1371/journal.pone.0035692
dc.relation.isreferencedby PMID:22545129
dc.subject Alpine species
dc.subject Foraging behaviour
dc.subject Diet
dc.subject DNA barcoding
dc.subject Habitat
dc.subject Predator-prey interactions
dc.title Data from: The foraging ecology of the Mountain long-eared bat Plecotus macrobullaris revealed with DNA mini-barcodes
dc.type Article *
dwc.ScientificName Plecotus macrobullaris
dwc.ScientificName Chiroptera
dwc.ScientificName Lepidoptera
dc.contributor.correspondingAuthor Alberdi, Antton
prism.publicationName PLoS ONE

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Title Analysed DNA sequences
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Description All the sequences analysed in the study in FASTA format. The name includes the MOTU number, the sequence identification number and the species or genus level identification.
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