Data from: Carrion fly-derived DNA as a tool for comprehensive and cost-effective assessment of mammalian biodiversity

 

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Title Short PCR product - all cycles with fusion primers
Downloaded 28 times
Description Sequencing of this amplicon was performed on a Pico Titer Plate using a GS FLX platform, according to manufacturer’s instructions (Roche Applied Sciences, Penzberg, Germany). Reads still comprise fusion primer sequences (forward 5'-CGTATCGCCTCCCTCGCGCCATCAGAGACGCACTCCGGTTGGGGTGACCTCGGA-3, reverse 5'-CTATGCGCCTTGCCAGCCCGCTCAGAGACGCACTCGCTGTTATCCCTAGGGTAACT-3').
Download mam_2_Fusion.fastq (17.36Mb)
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Title Short PCR product - last 5 cycles with fusion primers
Downloaded 28 times
Description Sequencing of this amplicon was performed on a Pico Titer Plate using a GS FLX platform, according to manufacturer’s instructions (Roche Applied Sciences, Penzberg, Germany). Reads still comprise fusion primer sequences (forward 5'-CGTATCGCCTCCCTCGCGCCATCAGAGACGCACTCCGGTTGGGGTGACCTCGGA-3, reverse 5'-CTATGCGCCTTGCCAGCCCGCTCAGAGACGCACTCGCTGTTATCCCTAGGGTAACT-3').
Download mam_2_2.Rd_Fusion_5x.fastq (18.38Mb)
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Title Long PCR product - all cycles with fusion primers
Downloaded 21 times
Description Sequencing of this amplicon was performed on a Pico Titer Plate using a GS FLX platform, according to manufacturer’s instructions (Roche Applied Sciences, Penzberg, Germany). Reads still comprise fusion primer sequences (forward 5'-CGTATCGCCTCCCTCGCGCCATCAGAGACGCACTCCGGTTGGGGTGACCTCGGA-3, reverse 5'-CTATGCGCCTTGCCAGCCCGCTCAGAGACGCACTCAGATAGAAACCGACCTGGAT-3').
Download mam_4.fastq (61.16Mb)
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Title Long PCR product - last 5 cycles with fusion primers
Downloaded 22 times
Description Sequencing of this amplicon was performed on a Pico Titer Plate using a GS FLX platform, according to manufacturer’s instructions (Roche Applied Sciences, Penzberg, Germany). Reads still comprise fusion primer sequences (forward 5'-CGTATCGCCTCCCTCGCGCCATCAGAGACGCACTCCGGTTGGGGTGACCTCGGA-3, reverse 5'-CTATGCGCCTTGCCAGCCCGCTCAGAGACGCACTCAGATAGAAACCGACCTGGAT-3').
Download mam_4_2.Rd_Fusion_5x.fastq (58.18Mb)
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Title Supporting Alignment S1
Downloaded 35 times
Description All Sanger sequences generated for this study.
Download Alignment_S1.fasta (135.4Kb)
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When using this data, please cite the original publication:

Calvignac-Spencer S, Merkel K, Kutzner N, Kühl H, Boesch C, Kappeler PM, Metzger S, Schubert G, Leendertz FH (2013) Carrion fly-derived DNA as a tool for comprehensive and cost-effective assessment of mammalian biodiversity. Molecular Ecology 22(4): 925-946. doi:10.1111/mec.12183

Additionally, please cite the Dryad data package:

Calvignac-Spencer S, Merkel K, Kutzner N, Kühl H, Boesch C, Kappeler PM, Metzger S, Schubert G, Leendertz FH (2013) Data from: Carrion fly-derived DNA as a tool for comprehensive and cost-effective assessment of mammalian biodiversity. Dryad Digital Repository. doi:10.5061/dryad.57vg4
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